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putR

transcriptional activator of the proline utilization operon [gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]

locus: BSU_03230

pI: 5.37

mw: 47.67 kDa

protein length: 411 aa

gene length: 1236 bp

function: regulation of proline utilization

product: transcriptional activator ([SW|PucR family])

essential: no

synonyms: ycgP,prcR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.2|Utilization of proline]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 348,724 → 349,959

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]

The protein

Catalyzed reaction/ biological activity

• transcription activation of the proline utilization operon ''[gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]'' in the presence of proline [Pubmed|21840319,21964733]

Protein family

• [SW|PucR family]
• [SW|CdaR family] (according to UniProt)

[SW|Cofactors]

• proline acts as co-activator [Pubmed|21840319]

Effectors of protein activity

• proline activates PutR [Pubmed|21840319]

Expression and Regulation

Operons

• genes: [gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]
  description: [Pubmed|21840319]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21964733], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • constitutively expressed [Pubmed|21840319,21964733]

Biological materials

Mutant

• BKE03230 (Δ[gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTTTCTCCTATAC, downstream forward: _UP4_TAAAAAAGAAACCAGTACTT
• BKK03230 (Δ[gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTTTCTCCTATAC, downstream forward: _UP4_TAAAAAAGAAACCAGTACTT

References

• 21840319,21964733,22139509