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dgcK
function
synthesis of c-di-GMP
product
diguanylate cyclase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
985,734 → 986,813
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-GMP from two molecules of GTP [Pubmed|23893111][SW|Domains]
six transmembrane helices at the N-terminus (according to UniProt?)contains a C-terminal [SW|GGDEF domain] (aa 223-357) [Pubmed|22821967]Structure
[PDB|2WB4] (the C-terminal [SW|GGDEF domain], PleD from Caulobacter vibrioides, 44% identity)[SW|Localization]
cell membrane at cell poles and septa, probably close to [protein|70ED3CE683F3A3F785480F40986CABE7729C17C7|YdaK] [pubmed|28536559]present at a single site in the cell membrane [pubmed|32156823]additional information
DgcK is present with about 6 molecules per cell [pubmed|32156823]Expression and Regulation
Operons
genes
[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-A656 (yhcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/656 NBRP B. subtilis, Japan]GP848 (''[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]''::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]BKE09120 (Δ[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTCBKK09120 (Δ[gene|C43AE224400421CCDA7022C915ECC7525E30EB1D|dgcK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTCReferences
23893111,22821967,27116468,28536559,32156823