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acoC [2018-02-10T12:44:14.000Z]
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acoC [2018-02-10T12:44:14.000Z]

acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
locus
BSU08080
pI
6.52
mw
42.73 kDa
protein length
398 aa Sequence Blast
gene length
1194 bp Sequence Blast
function
acetoin utilization
product
acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
essential
no
ec
2.3.1.12
synonyms
yfjI

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin]
  • Gene

    Coordinates
    881,049 → 882,245

    The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine (according to Swiss-Prot)
  • Protein family

  • lipoyl-binding domain (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|BkdB], [protein|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|OdhB], [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|PdhC]
  • [SW|Cofactors]

  • lipoic acid
  • [SW|Localization]

  • Membrane-proximal (Spotty) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
    description
    [pubmed|11274109]

    sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [PubMed|11274109], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]: activation, (interaction with [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]-containing [SW|RNA polymerase]) [ PubMed|11274109], in [regulon|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: indirect positive regulation, in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]
  • regulation

  • induced by acetoin ([protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]
  • additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|21815947]
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    Biological materials

    Mutant

  • MGNA-C282 (acoC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2280 NBRP B. subtilis, Japan]
  • BKE08080 (Δ[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC
  • BKK08080 (Δ[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC, downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC
  • Labs working on this gene/protein

  • [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
  • References

    Reviews

  • 27074917
  • Original publications

  • 11274109,10368162,10666464,16479537,16428414,12884008,21815947