SubtiBank SubtiBank
gabR

gabR

transcription activator of [gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], repressor of [gene|search|gabR ]([SW|MocR/ GabR family])
locus
BSU03890
pI
6.71
mw
55.00 kDa
protein length
479 aa Sequence Blast
gene length
1437 bp Sequence Blast
function
regulation of gamma-amino butyric acid utilization
product
transcription regulator
essential
no
synonyms
ycnF

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.10|Utilization of gamma-amino butyric acid]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • Gene

    Coordinates
    440,025 → 441,464

    The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the [gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD] operon in the presence of GABA and PLP, transcription repression of [gene|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR] [Pubmed|15223311]
  • Protein family

  • [SW|MocR/ GabR family] [Pubmed|22020104]
  • Paralogous protein(s)

  • none, but there are 7 members of the [SW|MocR/ GabR family] in B. subtilis
  • [SW|Domains]

  • N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]
  • C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574]
  • [SW|Cofactors]

  • gamma-aminobutyric acid, PLP [Pubmed|24127574]
  • Effectors of protein activity

  • activation of ''[gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate [Pubmed|15223311]
  • Structure

  • [PDB|4MGR] (the apo-protein) [Pubmed|24127574]
  • [PDB|4N0B] (the complex with PLP) [Pubmed|24127574]
  • Expression and Regulation

    Operons

    genes
    [gene|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR]
    description
    (according to [http://dbtbs.hgc.jp/COG/prom/gabR.html DBTBS]) null

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12123465], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR]: negative autoregulation, [Pubmed|12123465], in [regulon|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR regulon]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-C014 (ycnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2012 NBRP B. subtilis, Japan]
  • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • References

  • 11756427,15223311,12123465,24127574,22020104,25388514,25911692,27640111,26681693,28348215,28412355