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alr
function
peptidoglycan biosynthesis
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]Gene
Coordinates
517,372 → 518,541
Phenotypes of a mutant
reported to be essential [Pubmed|12682299], however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007) [Pubmed|17602222]auxotrophy for D-alaninea [gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA] [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr] double mutant is not viable due to the inability to acquire D-alanine [https://www.biorxiv.org/content/10.1101/562850v2 reference]The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)Protein family
alanine racemase family (with [protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|YncD], according to UniProt)Paralogous protein(s)
[protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|YncD] [SW|Cofactors]
PLP (according to UniProt)Structure
[PDB|1L6G] (with N-[5�-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus), [PDB|1SFT] (Geobacillus stearothermophilus)Expression and Regulation
Operons
genes
[gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]-[gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
[http://www.ncbi.nlm.nih.gov/nuccore/402479429?report=genbank&from=517383&to=518551 alr-1], two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in [http://www.bgsc.org/ BGSC] 1A4, a D-alanine auxotrophReferences
Ferrari E, Henner DJ, Yang MY. Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis, Nature Biotechnol 1985.Nov;3:1003-7.313801417602222