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mtnU
function
methionine salvage
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]Gene
Coordinates
1,424,767 → 1,425,546
Phenotypes of a mutant
reduced growth with 5-methylthioribose as single sulfur source [Pubmed|24837359]The protein
Catalyzed reaction/ biological activity
2-ketoglutaramate + H2O --→ 2-oxoglutarate + NH3 [Pubmed|24837359]Protein family
carbon-nitrogen hydrolase superfamily (with [protein|BDAA216E832B8EB267CED6456A0045254DD0E97D|YhcX], according to UniProt)[SW|Domains]
CN hydrolase (aa 3-238) (according to UniProt)Structure
[PDB|3P8K] (from Staphylococcus aureus, 42% identity)Expression and Regulation
Operons
genes
[gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]
description
[Pubmed|11545674]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]view in new tabBiological materials
Mutant
MGNA-A784 (ykrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/784 NBRP B. subtilis, Japan]BKE13570 (Δ[gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTAACACCCCAAA, downstream forward: _UP4_TAAAAAATATATTGACAACTBKK13570 (Δ[gene|CB12BCCF6CEADC0C64DD918E0BAE4748E358A67B|mtnU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTAACACCCCAAA, downstream forward: _UP4_TAAAAAATATATTGACAACTReferences
15102328,12022921,11545674,24837359