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ilvA
function
biosynthesis of branched-chain amino acids
product
threonine dehydratase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]Gene
Coordinates
2,292,769 → 2,294,037
The protein
Catalyzed reaction/ biological activity
L-threonine --> 2-oxobutanoate + NH4+ (according to UniProt)Protein family
serine/threonine dehydratase family (with [protein|4E7390A74A8261E8B732E94859E90FE256AD64EE|DsdA], according to UniProt)[SW|Domains]
ACT-like domain (aa 339-413) (according to UniProt)[SW|Cofactors]
PLP (according to UniProt)Structure
[PDB|1TDJ] (the enzyme from ''E. coli'', 39% identity) [Pubmed|9562556]Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]Expression and Regulation
Operons
genes
[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]-[gene|DE113BB32600349C472D3417B760D640B65B01AB|ypmP]
description
[Pubmed|15060025]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]regulation
repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]view in new tabBiological materials
Mutant
BKE21770 (Δ[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACAGATTCCTTTCTT, downstream forward: _UP4_TAATTTTTTTACACAACCGCBKK21770 (Δ[gene|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACAGATTCCTTTCTT, downstream forward: _UP4_TAATTTTTTTACACAACCGCExpression vector
pGP2289: expression of ''ilvA'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lablacZ fusion
pGP519 (in [SW|pAC5]), available in [SW|Stülke] labReferences
18083814,12618455,15060025,9562556,12618455,12107147,24163341,26220295