SubtiBank SubtiBank
lexA

lexA

transcriptional repressor of the SOS regulon
locus
BSU17850
pI
5.16
mw
22.70 kDa
protein length
205 aa Sequence Blast
gene length
615 bp Sequence Blast
function
regulation of DNA damage repair
product
transcriptional repressor of the SOS regulon
essential
no
synonyms
dinR

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • Gene

    Coordinates
    1,917,639 → 1,918,256

    The protein

    Catalyzed reaction/ biological activity

  • Represses ''[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB], [gene|DEB7605B4F6F5F365A49C1C15647F9767B01BF66|dinB], [gene|60344F56DEB167FA62C16E52E8238393CEE19312|tagC], [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]'' genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] interacts with [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA] causing an autocatalytic cleavage which disrupts the DNA-binding part of [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA], leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family

  • peptidase S24 family (according to Swiss-Prot)
  • Structure

  • [PDB|1JHH] (from ''E. coli'', S119A mutant, 33% identity, 52% similarity) [Pubmed|11551506]
  • Expression and Regulation

    Operons

    genes
    [gene|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]
    description
    [Pubmed|1657879]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|2548995], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • [protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|RocR]: autorepression, in [regulon|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|RocR regulon]
  • regulation

  • induced upon DNA damage ([protein|search|LexA]) [Pubmed|1657879,8899710,16267290]
  • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|fosB]' and '[protein|search|lexA]' [PubMed|20525796]
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    References

    Reviews

  • 22933559,18726173
  • Original publications

  • 8899710,9045831,9555905,8969214,1657879,16267290,11551506,16549676,20525796