folK

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

locus: BSU_00790

pI: 4.53

mw: 18.91 kDa

protein length: 167 aa

gene length: 504 bp

function: biosynthesis of folate

product: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

essential: yes

ec: 2.7.6.3

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 86,946 → 87,449

Phenotypes of a mutant

• essential [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• 6-hydroxymethyl-7,8-dihydropterin + ATP --> (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H⁺ (according to UniProt)

Protein family

• HPPK family (single member, according to UniProt)

Structure

• [PDB|1IM6] (from ''Escherichia coli'', 47% identity, 61% similarity) [Pubmed|12578370]

Expression and Regulation

Operons

• genes: [gene|B189AD3E3D18876E1956763182D835A151727392|pabB]-[gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]-[gene|FA9539CB701BC47FD4E849FBA6D7B201B27C6104|sul]-[gene|AAE44B4F73C7C02F7DB3574B6169E0DE46F4B787|folB]-[gene|D2B189C6292ACE1B25658B407C284E9A683EAE5B|folK]-[gene|F373EBC35805DC551E8AFF6FDC7E43D5587C4700|yazB]-[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]-[gene|24CC89DE55B3C19F80B11D106B9FD241174AE3E4|lysS]
  description: [Pubmed|9084182]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9084182], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • translation repressed by tryptophan ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]) [Pubmed|9084182]

References

Reviews

• 28031352

Original publications

• 9084182,28189581