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nadB
function
NAD biosynthesis
product
L-aspartate oxidase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
2,847,871 → 2,849,466
The protein
Catalyzed reaction/ biological activity
L-aspartate + O2 --> H2O2 + iminosuccinate (according to UniProt)Protein family
FAD-dependent oxidoreductase 2 family (with [protein|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|SdhA], according to UniProt)Modification
phosphorylated on Arg-104 [Pubmed|22517742][SW|Cofactors]
FAD (according to UniProt)Structure
[PDB|5KXJ] (from Salmonella typhimurium, 38% identity)Expression and Regulation
Operons
genes
[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]
description
[Pubmed|8444804]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8444804], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|16199587], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]regulation
repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]additional information
An [ncRNA|search|antisense RNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]view in new tabBiological materials
Mutant
BKE27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTGBKK27870 (Δ[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTGlabs
[SW|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]References
8444804,18959769,16199587,20525796,22517742