cotI

outer spore coat protein

locus: BSU_30920

pI: 4.99

mw: 41.09 kDa

protein length: 357 aa

gene length: 1074 bp

function: spore envelope

product: outer spore coat protein

essential: no

synonyms: ytaA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 3,162,084 → 3,163,157

The protein

Protein family

• cotS family (with [protein|DC99E507696A1CB23140A1CBA45DA07D1F7C7D53|CotS] and [protein|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|YutH], according to UniProt)

Structure

• [PDB|2Q83] [pubmed|20077512]

[SW|Localization]

• outer spore coat [pubmed|28870294]

Expression and Regulation

Operons

• genes: [gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]
  description: [Pubmed|12480901]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,12480901], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,12480901]

Biological materials

Mutant

• MGNA-A030 (ytaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/30 NBRP B. subtilis, Japan]
• BKE30920 (Δ[gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTACCTGTTTTACTC, downstream forward: _UP4_TAAGACCATCATTTCCCCCG
• BKK30920 (Δ[gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTACCTGTTTTACTC, downstream forward: _UP4_TAAGACCATCATTTCCCCCG

References

• 12562816,15699190,12480901,20077512,28870294