khtT [Thu Dec 15 2016 15:06:39 GMT+0100 (CET)]

K /H antiporter for K efflux

locus: BSU09860

pI: 4.00

mw: 18.00 kDa

function: potassium ion efflux

product: K /H antiporter for K efflux

essential: no

synonyms: yhaT

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence): 1,060,427 -> 1,060,924

The protein

[SW|Domains]

• contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:RCKC-terminaldomain* x])

[SW|Localization]

• cell membrane (peripheral) (according to Swiss-Prot)

[SW|Interactions]

• [protein|search|KhtT]-[protein|search|KhtU] [Pubmed|17679694]

Expression and Regulation

Operon

• ''[protein|search|khtS]-[protein|search|khtT]-[protein|search|khtU]'' [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]
• GP2078 (''[protein|search|khtS]-[protein|search|khtT]-[protein|search|khtU]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 14987767,17679694,24330391