SubtiBank SubtiBank
glcF

glcF

probable glycolate oxidase, iron-sulfur subunit
locus
BSU_28690
pI
6.45
mw
49.04 kDa
protein length
444 aa Sequence Blast
gene length
1335 bp Sequence Blast
function
unknown
product
probable glycolate oxidase, iron-sulfur subunit
essential
no
synonyms
ysfD, glcF

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,934,594 → 2,935,928

    The protein

    [SW|Domains]

  • 2 [SW|4Fe-4S ferredoxin-type domain]s (aa 14-46, aa 69-100) (according to UniProt)
  • [SW|Cofactors]

  • two Fe-S clusters [pubmed|29292548]
  • Expression and Regulation

    Operons

    genes
    [gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]-[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B005 (ysfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1004 NBRP B. subtilis, Japan]
  • BKE28690 (Δ[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATCTTTCCGCCACCAC, downstream forward: _UP4_TGAGAAAGCCCAAAACAGAC
  • BKK28690 (Δ[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATCTTTCCGCCACCAC, downstream forward: _UP4_TGAGAAAGCCCAAAACAGAC
  • References

  • 8969504,27264531,8606183