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amyE
function
starch degradation
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.23|Utilization of starch/ maltodextrin][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]Gene
Coordinates
327,618 → 329,597
The protein
Catalyzed reaction/ biological activity
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units (according to UniProt)Protein family
[SW|glycosyl hydrolase 13 family] (according to UniProt)Structure
[PDB|1BAG] (complex with maltopentaose)[SW|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]Expression and Regulation
Operons
genes
[gene|E48DE0C73CB859E2B367B6800E85BD94836390A5|ycgB]-[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
description
[Pubmed|22383849]
view in new tabgenes
[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]
description
[Pubmed|11320136]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3123701], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7665492], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon][protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|7665492]view in new tabBiological materials
Mutant
GP550 (cat), available in [SW|Stülke] labBKE03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGGBKK03040 (Δ[gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGGReferences
Reviews
Original publications
1904524,3123701,18957862,21948839,23262127,22900538,20817675,21512239,25431404