ysxC

assembly of the 50S subunit of the ribosome

locus: BSU_28190

pI: 9.71

mw: 21.88 kDa

protein length: 195 aa

gene length: 588 bp

function: ribosome assembly

product: GTPase

essential: yes

synonyms: orfX

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

Gene

Coordinates: 2,879,882 → 2,880,469

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• binds and hydrolyzes GTP and readily exchanges GDP for GTP
• participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]

Protein family

• [SW|TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|ThdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|Obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|YqeH], [protein|9F6B78C1932FB56F7F71430DB16BC862A312407B|YnbA], [protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|YphC], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|YyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|Era]

[SW|Domains]

• EngB-type G (aa 22-195) (according to UniProt)

Structure

• [PDB|1SVI] (complex with GDP) [pubmed|15136032]
• [PDB|1SUL] [pubmed|15136032]

[SW|Localization]

• cytosol (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]-[gene|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC]
  description: [Pubmed|7961402]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7961402], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

  regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]

Biological materials

Mutant

• MGNA-A996 (ysxC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/996 NBRP B. subtilis, Japan]

Expression vectors

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP834, available in [SW|Stülke] lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP842, available in [SW|Stülke] lab

labs

• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 19575570,21885683

Original publications

• 17981968,11073929,12427945,16997968,7961402,9852015,14684918,15136032,21636901,25771857,26102633,27484475