The second international online conference
#Subtillery2021 will be held 14th - 18th June - save the date, for more information see the
conference website!
The 21st
International Conference on Bacilli has been postponed to 2022 and will take place in Prague.
bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] autophosphorylation, and subsequently of entry into [SW|sporulation]
function
[SW|biofilm formation], control of entry into [SW|sporulation] via the [SW|phosphorelay]
product
biofilm surface layer, inhibitor of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] autophosphorylation
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.3|Repellent surface layer][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]Gene
Coordinates
3,187,503 → 3,188,048
Phenotypes of a mutant
reduced colony complexity [Pubmed|21742882]loss of surface repellency of the biofilm [Pubmed|22571672]diminished [SW|sliding] of ''B. subtilis'' natto [Pubmed|26152584]The protein
Catalyzed reaction/ biological activity
inhibits [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] autophophorylation [Pubmed|23335417]contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms [Pubmed|26873313]Protein family
BslA/BslB family (with [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|SivA], according to UniProt)Paralogous protein(s)
[protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|SivA] Structure
[PDB|4BHU] [Pubmed|23904481][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]biofilm matrix in an extracellular polysaccharide-dependent manner [Pubmed|22571672]forms a layer on the biofilm surface [Pubmed|22571672]Expression and Regulation
Operons
genes
[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]
description
[Pubmed|22383849]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18978066], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon][protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR]: activation, [Pubmed|24196425], in [regulon|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR regulon][protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|18978066], this might be indirect [Pubmed|21742882], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]view in new tabBiological materials
Mutant
MGNA-A218 (yuaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/218 NBRP B. subtilis, Japan]''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) [Pubmed|17850253]''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) [Pubmed|18978066]''B. subtilis'' ''bslA''::Cm (168 based) [Pubmed|21097620]BKE31080 (Δ[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAATTCCCCCTAA, downstream forward: _UP4_TAATGTAAAAGACCGGTTAABKK31080 (Δ[gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAATTCCCCCTAA, downstream forward: _UP4_TAATGTAAAAGACCGGTTAAExpression vectors
Physpank-bslA based on vector pDR111: pDRyuaB2 [Pubmed|21097620]lacZ fusion
pNW500 P''bslA-lacZ'' fusion in pDG1663 [Pubmed|18978066]GFP fusion
P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB [Pubmed|21097620]References
Reviews
22607588,23979433,24988880,25907113,27458867 Original publications
27298433,22571672,18978066,18957862,17850253,21097620,21742882,22383849,23335417,23904481,22571672,25870300,26152584,26378478,26873313,28698374,28701036,28751732,29623706