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response regulator aspartate phosphatase, controls [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity
function
control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity
product
response regulator aspartate phosphatase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]Gene
Coordinates
4,140,260 → 4,141,357
The protein
Catalyzed reaction/ biological activity
control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity [Pubmed|12950930]Protein family
[SW|RAP family] (according to UniProt)[SW|Domains]
six [SW|TPR repeat|tetratrichopeptide repeats] (according to UniProt)Effectors of protein activity
binding of [protein|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|PhrG] to [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG] prevents it from binding [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|12950930]Structure
[PDB|4I9E] ([protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF], 26% identity) [pubmed|23526880]Expression and Regulation
Operons
genes
[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]-[gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]
description
[Pubmed|12950930]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16553878], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon][protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|12950930], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon][protein|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR]: repression, [Pubmed|16553878], in [regulon|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR regulon][protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]regulation
repressed by glucose (4.8-fold) ([protein|search|CcpA]) [Pubmed|12850135]view in new tabBiological materials
Mutant
MGNA-B821 (yycM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1820 NBRP B. subtilis, Japan]BKE40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTATBKK40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTATReferences
16553878,16430695,12950930,23526880