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rapG

response regulator aspartate phosphatase, controls [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity

locus: BSU_40300

pI: 4.81

mw: 42.68 kDa

protein length: 365 aa

gene length: 1098 bp

function: control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity

product: response regulator aspartate phosphatase

essential: no

synonyms: yycM

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]

Gene

Coordinates: 4,140,260 → 4,141,357

The protein

Catalyzed reaction/ biological activity

• control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity [Pubmed|12950930]

Protein family

• [SW|RAP family] (according to UniProt)

[SW|Domains]

• six [SW|TPR repeat|tetratrichopeptide repeats] (according to UniProt)

Effectors of protein activity

• binding of [protein|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|PhrG] to [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG] prevents it from binding [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|12950930]

Structure

• [PDB|4I9E] ([protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF], 26% identity) [pubmed|23526880]

Expression and Regulation

Operons

• genes: [gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]-[gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]
  description: [Pubmed|12950930]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16553878], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|12950930], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR]: repression, [Pubmed|16553878], in [regulon|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose (4.8-fold) ([protein|search|CcpA]) [Pubmed|12850135]

Biological materials

Mutant

• MGNA-B821 (yycM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1820 NBRP B. subtilis, Japan]
• BKE40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTAT
• BKK40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTAT

References

• 16553878,16430695,12950930,23526880