menD

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase

locus: BSU_30820

pI: 5.66

mw: 63.92 kDa

protein length: 580 aa

gene length: 1743 bp

function: biosynthesis of menaquinone

product: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / 2-oxoglutarate decarboxylase

essential: yes

ec: 2.2.1.9

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,150,563 → 3,152,305

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + H⁺ + isochorismate --> 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO₂ (according to UniProt)

Protein family

• [SW|TPP enzyme family] (according to UniProt)

[SW|Cofactors]

• thiamine pyrophosphate, divalent metal ion such as Mn(2+)) [Pubmed|20600129]

Structure

• [PDB|2X7J] [Pubmed|20600129]

Expression and Regulation

Operons

• genes: [gene|8EFE4D2B07E54A0EE7D6821C9FEBFC7DC22FDE03|menF]-[gene|E3283812B7EB374EAC8722ECCE28432DE24B7B4C|menD]-[gene|74659B6794496912E139B3CF9EFF787B63729A3F|ytxM]-[gene|224584953B0BC1AF666CD69372B2D53AC56F6752|menB]-[gene|16412BAB04BBF9FD1C3653328923687874C81868|menE]-[gene|9B2F45BC37D4F356B366447A33DC95639EF484EE|menC]
  description: [Pubmed|9387221,1629163,8550504]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8550504], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]

References

• 9387221,1629163,8550504,20600129