tagF

CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase

locus: BSU_35720

pI: 9.40

mw: 87.87 kDa

protein length: 746 aa

gene length: 2241 bp

function: biosynthesis of teichoic acid

product: CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase

essential: yes

ec: 2.7.8.12

synonyms: rodC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,676,159 → 3,678,399

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 4-O-[(2R)-glycerylphospho]-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + n CDP-glycerol --> 4-O-{[(2R)-1-glycerylphospho]_(n)-(2R)-1-glycerylphospho}-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl undecaprenyl diphosphate + n CMP + n H⁺ (according to UniProt)

Protein family

• CDP-glycerol glycerophosphotransferase family (with [protein|911A4D957A6C0DFC636A152395119DBFC777DDA2|TagB], according to UniProt)

Structure

• [PDB|3L7I] (C-terminal domain of the Staphylococcus epidermidis protein, 48% identity) [pubmed|20400947]

[SW|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD]-[gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]-[gene|E730E41546298960A4B907FCB7F36C495AA9602B|tagF]-[gene|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|tagG]-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7581998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: repression, [Pubmed|9457886], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • repressed by phosphate ([protein|search|PhoP]) [Pubmed|9457886]

References

Reviews

• 24024634

Original publications

• 18156271,16141206,2507871,12637499,1712357,9457886,18763711,19520862,7581998,12950927,15547257,23033921,22383849,20400947