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The 21st
International Conference on Bacilli has been postponed to 2022 and will take place in Prague.
product
elongation factor Tu
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]Gene
Coordinates
132,882 → 134,072
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Protein family
[SW|TRAFAC class translation factor GTPase superfamily] (according to UniProt)[SW|Classic translation factor GTPase family] (according to UniProt)[SW|Domains]
[SW|tr-type G domain] (aa 10-205) (according to UniProt)Modification
phosphorylated on Thr-384 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|19246764], [Pubmed|17726680]Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [Pubmed|22938038]phosphorylated on Arg-265 [Pubmed|31221751]phosphorylated on several Arg residues [Pubmed|24263382]phosphorylated during [SW|sporulation] on Thr-63 by [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|YabT], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|26056311]phosphorylated on Tyr-270 [Pubmed|26381121]Effectors of protein activity
phosphorylation by YabT results in inhibtion of GTP hydrolysis; as a result, EF-Tu remains stably boundto the [SW|ribosome], stalling protein [SW|translation] [Pubmed|26056311]Structure
[PDB|3U6B] (from ''E. coli '', 77% identity) [Pubmed|21999529][SW|Localization]
EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [Pubmed|20133608]membrane [Pubmed|18763711]cytoplasm (according to UniProt)additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Expression and Regulation
Operons
genes
[gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
description
[pubmed|22383849]
regulatory mechanism
[regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]view in new tabBiological materials
Expression vector
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP839, available in [SW|Stülke] labfor expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP847, available in [SW|Stülke] labtwo-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s labReferences
22938038,19246764,20133608,25707802,19246764,17726680,18763711,24263382,15378759,25676310,26056311,26381121,11918677,28516784,30394869,31221751,32024753,32949815