SubtiBank SubtiBank
lytF [Tue Feb 23 2016 18:24:00 GMT+0100 (CET)]
SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

lytF [Tue Feb 23 2016 18:24:00 GMT+0100 (CET)]

gamma-D-glutamate-meso-diaminopimelate muropeptidase, cell separase (major autolysin)
locus
BSU09370
pI
10.00
mw
51.00 kDa
function
cell separation
product
gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
essential
no
ec
3.4.-.-
synonyms
cwlE, yhdD

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases]
  • [SW|Categories] containing this gene/protein

  • [SW|cell wall degradation/ turnover]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigD regulon], [SW|SlrR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,011,792 -> 1,013,258

    Phenotypes of a mutant

  • cell separation defect, this is increased by a ''[protein|search|lytE]'' mutation [Pubmed|23855774]
  • The protein

    Protein family

  • nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s)

  • the C-terminal D,L-endopeptidase domains of [protein|search|LytE], [protein|search|LytF], [protein|search|CwlS], and [protein|search|CwlO] exhibit strong sequence similarity
  • [SW|Domains]

  • contains five N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
  • C-terminal D,L-endopeptidase domain [Pubmed|22139507]
  • [SW|Localization]

  • secreted (according to Swiss-Prot)
  • localizes to cell septa and poles on the vegetative cell surface, this depends on the presence of lipoteichoic acids ([protein|search|LtaS]) [Pubmed|25288647,22139507]
  • [SW|Interactions]

  • [protein|search|IseA]-[protein|search|LytF] (catalytic domain) [Pubmed|18761696]
  • Expression and Regulation

    Operon

  • ''[protein|search|lytF]'' [Pubmed|10322020]
  • [SW|Sigma factor]

  • [protein|search|SigD] [Pubmed|10322020]
  • Regulatory mechanism

  • [protein|search|SlrR] (in complex with [protein|search|SinR]: transcription repression [Pubmed|20351052]
  • Biological materials

    Mutant

  • 1A791 ( ''lytF''::''spec''), [Pubmed|10206711], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A791&Search=1A791 BGSC]
  • 1A792 ( ''lytF''::''spec''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
  • References

    Reviews

  • 18430080
  • Original publications

  • 20351052,10322020,10206711,14594841,18761696,19542270,22139507,23855774,23091053,25288647