SubtiBank SubtiBank
spoVG
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spoVG

RNA-binding regulatory protein, negative effector of asymetric septation at the onset of sporulation
locus
BSU_00490
pI
5.11
mw
10.75 kDa
protein length
gene length
294 bp Sequence Blast
function
cell division, control of sporulation initiation
product
negative effector of asymetric septation
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    55,866 → 56,159

    The protein

    Protein family

  • SpoVG family (single member, according to UniProt)
  • Modification

  • phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) [Pubmed|17218307]
  • Structure

  • [PDB|2IA9]
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]
    description
    [Pubmed|408013]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|408013], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|2437099], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|16430695]
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    Biological materials

    Mutant

  • MGNA-A082 (spoVG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/82 NBRP B. subtilis, Japan]
  • GP2109 (''[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]''::''tet'', available in [SW|Jörg Stülke]'s lab)
  • BKE00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
  • BKK00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT, downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
  • Expression vector

  • pGP2331: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab
  • Expression vectors

  • pGP2163: expression of SpoVG-Strep by [SW|pGP380] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP2310: expression of SpoVG-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • References

  • 408013,1850083,16430695,6405278,6411929,10348850,7559352,8419299,3100810,2437099,17218307,20803133,27048798