glpT

glycerol-3-phosphate permease

locus: BSU_02140

pI: 9.37

mw: 49.64 kDa

protein length: 444 aa

gene length: 1335 bp

function: glycerol-3-phosphate uptake

product: glycerol-3-phosphate permease

essential: no

synonyms: ybeE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.3|Carbohydrate transporter]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.3|Utilization of glycerol/ glycerol-3-phosphate]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.1|Utilization of phospholipids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 233,994 → 235,328

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)

Structure

• [PDB|1PW4] (from E. coli, 60% identity) [pubmed|12893936]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]-[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]
  description: [Pubmed|8012593]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8012593], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP]: antitermination, via a protein-dependent [SW|RNA switch] [Pubmed|8012593], in [regulon|38767691AE7E09F46B9E97A60BF5358C1876EDF8|GlpP regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|10913081], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by glycerol ([protein|search|GlpP]) [Pubmed|8012593]

Biological materials

Mutant

• QB5437 (ermC), available in the [SW|Stülke] lab
• BKE02140 (Δ[gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA
• BKK02140 (Δ[gene|ECCBD8EF461C24D693450E10EC268DBC7A440618|glpT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATAATTATCCCCCTT, downstream forward: _UP4_TAAGAAAGACACATAAAAGA

References

• 8012593,12850135,12893936