SubtiBank SubtiBank
hutU
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hutU

urocanase
locus
BSU_39360
pI
5.66
mw
60.43 kDa
protein length
552 aa Sequence Blast
gene length
1659 bp Sequence Blast
function
histidine utilization
product
urocanase
essential
no
ec
4.2.1.49
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.5|Utilization of histidine]
  • Gene

    Coordinates
    4,043,574 → 4,045,232

    The protein

    Catalyzed reaction/ biological activity

  • 4-imidazolone-5-propanoate --> H2O + trans-urocanate (according to UniProt)
  • Protein family

  • urocanase family (single member, according to UniProt)
  • Structure

  • [PDB|2FKN]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]-[gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH]-[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]-[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]-[gene|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG]-[gene|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8071225], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8071225], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|8682780], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP]: antitermination, at a protein-dependent [SW|RNA switch] located between [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP] and [gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH] [Pubmed|8071225], in [regulon|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP regulon]
  • regulation

  • induced by histidine ([protein|search|HutP]) [Pubmed|8071225]
  • view in new tab

    Biological materials

    Mutant

  • BKE39360 (Δ[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCATATGTTCATCCCCCT, downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA
  • BKK39360 (Δ[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCATATGTTCATCCCCCT, downstream forward: _UP4_ATGCAAAAATAAAGGAGTGA
  • References

    Reviews

  • 22933560
  • Original publications

  • 10746760,8071225,8682780,10217782,21821766