SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

gerKA

nutrient receptor

locus: BSU_03700

pI: 5.21

mw: 60.22 kDa

protein length: 544 aa

gene length: 1635 bp

function: germination response to the combination of glucose, fructose, aspartate, and KCl

product: nutrient receptor

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.1|Germinant receptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 420,110 → 421,744

The protein

Protein family

• [SW|GerABKA family] (according to UniProt)

Paralogous protein(s)

• [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|GerAA], [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|YndD], [protein|3368743E6E03792DB83A38A19989123304DF7560|GerBA], [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|YfkQ]

[SW|Domains]

• contains multiple membrane-spanning domains [Pubmed|23335419]

Structure

• [PDB|6O59] (from B. megaterium, corresponds to aa 40 ... 299, 53.6% identity) [pubmed|31113879]

[SW|Localization]

• outer surface of the inner spore membrane [Pubmed|23335419,21696470]

Additional information

• 700 molecules are present per spore [Pubmed|23749970]

Expression and Regulation

Operons

• genes: [gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[gene|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC]-[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]
  description: [pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16707705], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, [Pubmed|16707705], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

  regulation

  • expressed during sporulation ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|16707705]

  additional information

  • 700 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
• BKK03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA

References

• 16707705,16352818,21696470,23335419,23396907,22343299,16740944,23749970,24752279,26731423,31113879