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gerKA
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gerKA

nutrient receptor
locus
BSU_03700
pI
5.21
mw
60.22 kDa
protein length
544 aa Sequence Blast
gene length
1635 bp Sequence Blast
function
germination response to the combination of glucose, fructose, aspartate, and KCl
product
nutrient receptor
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.1|Germinant receptors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    420,110 → 421,744

    The protein

    Protein family

  • [SW|GerABKA family] (according to UniProt)
  • Paralogous protein(s)

  • [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|GerAA], [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|YndD], [protein|3368743E6E03792DB83A38A19989123304DF7560|GerBA], [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|YfkQ]
  • [SW|Domains]

  • contains multiple membrane-spanning domains [Pubmed|23335419]
  • Structure

  • [PDB|6O59] (from B. megaterium, corresponds to aa 40 ... 299, 53.6% identity) [pubmed|31113879]
  • [SW|Localization]

  • outer surface of the inner spore membrane [Pubmed|23335419,21696470]
  • Additional information

  • 700 molecules are present per spore [Pubmed|23749970]
  • Expression and Regulation

    Operons

    genes
    [gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[gene|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC]-[gene|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16707705], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, [Pubmed|16707705], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]
  • regulation

  • expressed during sporulation ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG], [SW|SpoVT]) [Pubmed|16707705]
  • additional information

  • 700 molecules are present per spore [PubMed|23749970]
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    Biological materials

    Mutant

  • BKE03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
  • BKK03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT, downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
  • References

  • 16707705,16352818,21696470,23335419,23396907,22343299,16740944,23749970,24752279,26731423,31113879