SubtiBank SubtiBank
znuB [2018-05-28 18:06:11]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

znuB [2018-05-28 18:06:11]

high-affinity ABC transporter for zinc (permease)
locus
BSU02870
pI
10.38
mw
30.28 kDa
protein length
280 aa Sequence Blast
gene length
840 bp Sequence Blast
function
zinc uptake
product
ABC transporter for zinc (permease)
essential
no
synonyms
yceA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    310,000 → 310,842

    Phenotypes of a mutant

  • reduced genetic competence, can be rescued by the addition of excess zinc [Pubmed|21813502]
  • reduced expression of the ''[gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]'' operon [Pubmed|21813502]

    • The protein

    Protein family

  • ABC-3 integral membrane protein family (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]-[gene|6B5D4FD32CCE72C99B915B0F1EB4B8E8D42A04B3|znuC]-[gene|EE212953BC83BD6D69BF769D6E917F4F954BE1E2|znuB]
    description
    [Pubmed|9811636]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

    • regulation

  • induced by zinc starvation ([protein|search|Zur]) [Pubmed|12426338]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B982 (yceA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1981 NBRP B. subtilis, Japan]
  • BKE02870 (Δ[gene|EE212953BC83BD6D69BF769D6E917F4F954BE1E2|znuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGGCGATCATGCCGC, downstream forward: _UP4_TAACATGAAAAGCAGTTTTC
  • BKK02870 (Δ[gene|EE212953BC83BD6D69BF769D6E917F4F954BE1E2|znuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACGGCGATCATGCCGC, downstream forward: _UP4_TAACATGAAAAGCAGTTTTC

    • References

  • 12426338,9811636,10092453,21813502,12426338,27561249