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The 21st
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ytpR
similar to Phe-tRNA synthetase (beta subunit)
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]Gene
Coordinates
3,052,743 → 3,053,348
The protein
[SW|Domains]
[SW|tRNA-binding domain] (aa 90-200) (accordinig to UniProt)Structure
[PDB|3BU2] (tRNA-binding protein from Staphylococcus saprophyticus, 59% identity)Expression and Regulation
Operons
genes
[gene|314D5C01B4BDDC3B122F6D4066647B143B2A6543|ytpP]-[gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]-[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]
description
[Pubmed|21949854]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21949854], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|21949854], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]view in new tabBiological materials
Mutant
MGNA-A512 (ytpR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/512 NBRP B. subtilis, Japan]BKE29820 (Δ[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGTTCATAAGTAAAAATC, downstream forward: _UP4_TAGACTCCCATAAATCCGCCBKK29820 (Δ[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGTTCATAAGTAAAAATC, downstream forward: _UP4_TAGACTCCCATAAATCCGCCReferences