SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

narK

nitrite extrusion protein

locus: BSU_37320

pI: 10.10

mw: 42.80 kDa

protein length: 395 aa

gene length: 1188 bp

function: nitrate respiration, nitrite export

product: nitrite extrusion protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.11|Other exporters]
• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,832,327 → 3,833,514

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)
• Nitrate/nitrite porter (TC 2.A.1.8) family (with [protein|2DE7BEC614129384779F4C761E0792385DECC563|NasA], according to UniProt)

Paralogous protein(s)

• [protein|2DE7BEC614129384779F4C761E0792385DECC563|NasA]

Structure

• [PDB|4JR9] (from E. coli, 25% identity) [pubmed|23665960]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]-[gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]
  description: [Pubmed|8846791]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|16885456], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • ''[protein|search|fnr]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

Biological materials

Mutant

• BKE37320 (Δ[gene|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCTTGCCCCTTTCA, downstream forward: _UP4_TAACACTGGGGCATTCACAA
• BKK37320 (Δ[gene|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGCTTGCCCCTTTCA, downstream forward: _UP4_TAACACTGGGGCATTCACAA

References

Reviews

• 11289299

Original publications

• 16885456,8846791,16428414,18763711,23665960