rpsB

ribosomal protein

locus: BSU_16490

pI: 6.00

mw: 27.00 kDa

protein length: 246 aa

gene length: 741 bp

function: translation

product: ribosomal protein S2

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

Gene

Coordinates: 1,717,933 → 1,718,673

Phenotypes of a mutant

• essential [Pubmed|23002217,12682299]

The protein

Protein family

• universal [SW|ribosomal protein] uS2 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

[SW|Localization]

• [SW|ribosome], at the dimer interface of hibernating ribosomes to prevent loss of the protein [pubmed|32123037]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [Pubmed|11948165], in [regulon|stringent response|stringent response]
  • [protein|F1969E4C7BAAF70BCBE570F58350C12A3E417539|RpsB]: autorepression, [pubmed|32448158], in [regulon|F1969E4C7BAAF70BCBE570F58350C12A3E417539|RpsB regulon]

  regulation

  • autorepression of ''rpsB'' expression upon binding of excess [protein|F1969E4C7BAAF70BCBE570F58350C12A3E417539|RpsB] to a [SW|RNA switch] in the untranslated region of the ''rpsB'' mRNA [Pubmed|32448158]
  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

• genes: [gene|F1969E4C7BAAF70BCBE570F58350C12A3E417539|rpsB]-[gene|94E1130A787CBCCCDBDA93C8AFC24ECB82D5FE61|tsf]-[gene|40655595D0C573B4CB75FB255CBB01A6ACF1F94D|pyrH]-[gene|093960C48AD50C0D0B3A68BBFE7D060BE716935D|frr]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

lacZ fusion

• pGP602 (in [SW|pAC6]), available in [SW|Stülke] lab

References

• 18763711,19653700,23002217,15378759,25903689,27120414,28468753,28516784,32123037