SubtiBank SubtiBank
ykoY
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ykoY

membrane protein, may be involved in manganese detoxification
locus
BSU13440
pI
9.73
mw
35.38 kDa
protein length
324 aa Sequence Blast
gene length
972 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    1,410,654 → 1,411,628

    Expression and Regulation

    Operons

    genes
    [gene|F39BC9CA68DD8E80D3ED021272A6F54EA9C57C7B|ykoY]
    description
    [Pubmed|25794618]

    regulatory mechanism

  • [regulon|yybP-ykoY motif|yybP-ykoY motif]: antitermination, via riboswitch in the presence of the ligand Mn2+ [Pubmed|25794618], in [regulon|yybP-ykoY motif|yybP-ykoY motif]
  • regulation

  • induced in the presence of Mn2+ ([[yybP-ykoY motif]]) [Pubmed|25794618]
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    Biological materials

    Mutant

  • MGNA-A780 (ykoY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/780 NBRP B. subtilis, Japan]
  • BKE13440 (Δ[gene|F39BC9CA68DD8E80D3ED021272A6F54EA9C57C7B|ykoY]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGACGCTACTCCCC, downstream forward: _UP4_TAATCTGAAAGACTCTGCTT
  • BKK13440 (Δ[gene|F39BC9CA68DD8E80D3ED021272A6F54EA9C57C7B|ykoY]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGGACGCTACTCCCC, downstream forward: _UP4_TAATCTGAAAGACTCTGCTT
  • References

  • 15096624,25794618,25794619