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gcvT
aminomethyltransferase (glycine cleavage system protein T)
function
glycine utilization
product
aminomethyltransferase (glycine cleavage system protein T)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]Gene
Coordinates
2,548,245 → 2,549,333
The protein
Catalyzed reaction/ biological activity
(6S)-5,6,7,8-tetrahydrofolate + (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[protein] --> (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N6-dihydrolipoyl-L-lysyl-[protein] + NH4+ (according to UniProt)Protein family
gcvT family (single member, according to UniProt)[SW|Cofactors]
tetrahydrofolateStructure
[PDB|1YX2]Expression and Regulation
Operons
genes
[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]-[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]
description
[Pubmed|15472076]
regulatory mechanism
[regulon|Gly-box|Gly-box]: termination, [pubmed|31992591], in [regulon|Gly-box|Gly-box]regulation
induced by glycine [Pubmed|15472076]the [SW|Gly-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]view in new tabBiological materials
Mutant
MGNA-C428 (yqhI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2426 NBRP B. subtilis, Japan]BKE24570 (Δ[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA, downstream forward: _UP4_TAATATTTTTTCTTGGAGAGBKK24570 (Δ[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA, downstream forward: _UP4_TAATATTTTTTCTTGGAGAGlacZ fusion
pGP431 (in [SW|pAC7]), available in [SW|Stülke] labReferences
15096624,15472076,23249744,23721735,29794222,29089431,31992591