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dnaG
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dnaG

DNA primase, part of the [SW|replisome]
locus
BSU_25210
pI
6.71
mw
68.62 kDa
protein length
603 aa Sequence Blast
gene length
1812 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication]
product
DNA primase
essential
yes
ec
2.7.7.-
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    2,601,528 → 2,603,339

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • required for bacteriophage SPP1 replication [Pubmed|23268446]

    • Protein family

  • DNA primase family (single member, according to UniProt)

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 262-343) [Pubmed|9722641]

    • Effectors of protein activity

  • the enzymatic activity is inhibited by (p)ppGpp during the 'stringent response' [Pubmed|17350574]
  • [protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] primase activity is stimulates by [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC] [Pubmed|23563155]

    • Structure

  • [PDB|1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
  • [PDB|1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
  • [PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|DnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|DnaG] complex) [Pubmed|24048025]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
    description
    [Pubmed|3298998]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

    • additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]
    • view in new tab

    References

    Reviews

  • 22797751,17350566,27149325,28075389

    • Original publications

  • 3919021,20122408,14651647,16479537,19415803,17350574,23563155,23268446,21419346,24048025,9849877,9722641,28386108,28575448