rplK [2019-07-08 10:38:19]

ribosomal protein

locus: BSU01020

pI: 9.72

mw: 14.78 kDa

protein length: 141 aa

gene length: 426 bp

function: translation

product: ribosomal protein L11 (BL11)

essential: no

synonyms: relC,tsp

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

Gene

Coordinates: 118,591 → 119,016

Phenotypes of a mutant

• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

The protein

Protein family

• Universal ribosomal protein uL11 family (single member, according to UniProt)

Modification

• phosphorylated on Arg-94 [Pubmed|22517742]

Structure

• [PDB|1FOX] (C-terminal domain, Geobacillus stearothermophilus)
• [PDB|2FOW] (RNA binding domain in complex with RNA, Geobacillus stearothermophilus)
• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

[SW|Localization]

• [SW|ribosome] (according to UniProt)

Biological materials

Mutant

• BKE01020 (Δ[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT
• BKK01020 (Δ[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGACACACCTCCTTAA, downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT

References

• 11244072,11948165,19653700,20441189,20525796,22517742,112097,23002217,25903689,28516784,28189581