SubtiBank SubtiBank
polC
The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

polC

DNA polymerase III, leading strand polymerase (alpha subunit), part of the [SW|replisome]
locus
BSU_16580
pI
5.14
mw
162.42 kDa
protein length
1437 aa Sequence Blast
gene length
4311 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication]
product
DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms
mutI,dnaF,dnaP

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,727,133 → 1,731,446

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for bacteriophage SPP1 replication [Pubmed|23268446]
  • Protein family

  • DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|YorL] and [protein|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|DnaE], according to UniProt)
  • [SW|Domains]

  • [SW|Exonuclease domain] (aa 421-578) (according to UniProt)
  • Structure

  • [PDB|3F2B] (from ''Geobacillus kaustophilus'', 71% identity) [Pubmed|19106298]
  • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • about three molecules of PolC at each replication fork [pubmed|28002733]
  • Expression and Regulation

    Operons

    genes
    [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
    view in new tab

    additional information

  • about 60 molecules per cell [pubmed|28002733]
  • References

    Reviews

  • 19106294,28073967,29197672
  • Original publications

  • 15186423,20122408,11721055,23563155,23268446,21740522,21958350,24062730,24106089,19106298,28002733,28575448,28878042,30686488,31251806