ytdA

similar to UTP-glucose-1-phosphate uridylyltransferase

locus: BSU_30850

pI: 5.91

mw: 30.03 kDa

protein length: 272 aa

gene length: 819 bp

function: unknown

product: unknown

essential: no

ec: 2.7.7.9

synonyms: yzwA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,154,735 → 3,155,553

The protein

Catalyzed reaction/ biological activity

• α-D-glucose 1-phosphate + H⁺ + UTP --> diphosphate + UDP-α-D-glucose (according to UniProt)

Protein family

• UDPGP type 2 family (with [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB] and [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|YngB], according to UniProt)

Paralogous protein(s)

• [protein|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|YngB], [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB]

Structure

• [PDB|3JUJ] (UDP-glucose pyrophosphorylase from ''Helicobacter pylori'', 43% identity) [Pubmed|20238176]

[SW|Localization]

• outer spore coat [Pubmed|26577401]

Expression and Regulation

Operons

• genes: [gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during [SW|sporulation] in the mother cell ([SW|SigK]) [Pubmed|26577401]

Biological materials

Mutant

• MGNA-A281 (ytdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/281 NBRP B. subtilis, Japan]
• BKE30850 (Δ[gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC, downstream forward: _UP4_AAGAACCCCCAATAATTGGG
• BKK30850 (Δ[gene|FC455B727F66632D94D93E103B30748944ED04A0|ytdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC, downstream forward: _UP4_AAGAACCCCCAATAATTGGG

References

• 26577401,20238176,22383849