SubtiBank SubtiBank
dprA
SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

dprA

conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]; facilitates the displacement of both SSBs ([protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]), increases [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU_16110
pI
9.76
mw
32.78 kDa
protein length
297 aa Sequence Blast
gene length
894 bp Sequence Blast
function
[category|SW 3.1.5|DNA repair/ recombination]
product
recombination mediator protein
essential
no
synonyms
smf

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • Gene

    Coordinates
    1,682,580 → 1,683,473

    Phenotypes of a mutant

  • strongly impaired genetic recombination [Pubmed|23779106]
  • ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
  • loss of plasmid transformation [pubmed|30050509]
  • The protein

    Catalyzed reaction/ biological activity

  • binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] from ssDNA [Pubmed|23779106]
  • provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]) [Pubmed|22373918]
  • [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]
  • the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] onto any fragmented linear SPP1 ssDNA [pubmed|31876108]
  • anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
  • [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]
  • Protein family

  • DprA/Smf family (single member, according to UniProt)
  • Structure

  • [PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]
  • [SW|Localization]

  • transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]
  • Expression and Regulation

    Operons

    genes
    [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • regulation

  • repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|12850135]
  • expression is heterogeneous [pubmed|29809139]
  • view in new tab

    additional information

  • expressed under conditions that trigger genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]) [Pubmed|11948146]
  • Biological materials

    Mutant

  • BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
  • BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16110 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
  • labs

  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
  • References

    Reviews

  • 31950915
  • Original publications

  • 17630974,17803906,12421306,18045469,11948146,22373918,23779106,23440217,25138221,26786319,28618091,22904190,28911099,30050509,31876108