SubtiBank SubtiBank
fabHB
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fabHB

beta-ketoacyl-acyl carrier protein synthase III
locus
BSU_10170
pI
5.68
mw
35.27 kDa
protein length
325 aa Sequence Blast
gene length
978 bp Sequence Blast
function
fatty acid biosynthesis
product
beta-ketoacyl-acyl carrier protein synthase III
essential
no
ec
2.3.1.180
synonyms
yhfB

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • Gene

    Coordinates
    1,092,770 → 1,093,747

    The protein

    Catalyzed reaction/ biological activity

  • acetyl-CoA + H+ + malonyl-[ACP] --> 3-oxobutanoyl-[ACP] + CO2 + CoA (according to UniProt)
  • Protein family

  • [SW|thiolase-like superfamily] (according to UniProt)
  • Paralogous protein(s)

  • [protein|40E064365588CFED483FBFED02BFC0DA531CCC4A|FabHA]:
  • Structure

  • [PDB|1EBL] (FabH from ''E. coli'') [Pubmed|10673437]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis [Pubmed|19820084]
  • Expression and Regulation

    Operons

    genes
    [gene|FC818B3A8A3080231C1F8DE95377325C8E754DCE|fabHB]
    description
    [Pubmed|12737802]

    regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]
  • regulation

  • induced if the cells experience a lack of malonyl-CoA ([protein|search|FapR]) [Pubmed|12737802]
  • view in new tab

    Biological materials

    Mutant

  • BKE10170 (Δ[gene|FC818B3A8A3080231C1F8DE95377325C8E754DCE|fabHB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATCACTCCTTATG, downstream forward: _UP4_TAACAAAAAAAGAACTTGTT
  • BKK10170 (Δ[gene|FC818B3A8A3080231C1F8DE95377325C8E754DCE|fabHB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAATCACTCCTTATG, downstream forward: _UP4_TAACAAAAAAAGAACTTGTT
  • References

    Reviews

  • 15952903,17919287
  • Original Publications

  • 12737802,17114254,10629181,10673437,19820084,21383089,25196128,30925337