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leuS

Leu-tRNA synthetase

locus: BSU30320

pI: 4.86

mw: 91.35 kDa

protein length: 804 aa

gene length: 2412 bp

function: translation

product: leucyl-tRNA synthetase

essential: yes

ec: 6.1.1.4

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,102,629 → 3,105,043

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)

Protein family

• class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Structure

• [PDB|2AJG] (from ''Escherichia coli'', 39% identity, 56% similarity) [Pubmed|16277600]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|FEC89C6F5A825D925D730965ACBAA06EC5CC4E44|leuS]
  description: [Pubmed|1317842]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, via uncharged tRNA-Leu binding to [SW|RNA switch], in [regulon|T-box|T-box]

  regulation

  • induced by leucine limitation ([protein|search|T-box]) [Pubmed|1317842]

References

Reviews

• 19258532

Original Publications

• 1317842