ispF

ispF
168

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

locus
BSU_00910
Molecular weight
16.98 kDa
pI
5.4
Protein length
Gene length
function
MEP pathway of isoprenoid biosynthesis
product
2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
essential
yes
ec
4.6.1.12
synonyms
ispF, yacN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0245

This gene is a member of the following regulons

Gene
Coordinates
110,480  110,956
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
4-CDP-2-C-methyl-D-erythritol 2-phosphate --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP (according to UniProt)
Protein family
IspF family (single member, according to UniProt)
Structure
[PDB|5IWX] (native) [Pubmed|29335298]
[PDB|5IWY] (complex with CMP) [Pubmed|29335298]
[AF|Q06756]
Expression and Regulation
Operons
genes
[gene|179FDEA51A060DF32A3EA06A796793DE27B600FF|yacL]-[gene|CF9633F769263C6D16EE778F6BD2ECF1995F512A|ispD]-[gene|1A6793A3A5AC568C67873C8708E9F581F42AB9BA|ispF]
description
[Pubmed|10482513]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
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[gene|179FDEA51A060DF32A3EA06A796793DE27B600FF|yacL]→[gene|1A6793A3A5AC568C67873C8708E9F581F42AB9BA|ispF]

2022-11-24 18:53:40

ghost

30

7dc686870ed58f71ca9e5b0841c69a922f74d84a

EFDC5F8C8F63B342C29A9AB39CD8005147D16917

Biological materials
Mutant
MGNA-B882 (yacN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1881 NBRP B. subtilis, Japan]
References
15805528,10482513,12270818,17458547,23840410,15502301,29335298

1A6793A3A5AC568C67873C8708E9F581F42AB9BA

Page visits: 3680

Time of last update: 2022-12-01 17:59:20

Author of last update: Melvin.boenninger