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accC
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accC
168

acetyl-CoA carboxylase (biotin carboxylase subunit)

locus
BSU_24340
Molecular weight
49.41 kDa
pI
4.72
Protein length
Gene length
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxylase subunit)
essential
yes
ec
6.3.4.14
synonyms
accC, yqhX

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0439

This gene is a member of the following regulons

Gene
Coordinates
2,530,354  2,531,706
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] --> ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
acetyl-CoA + ATP + hydrogencarbonate --> ADP + H+ + malonyl-CoA + phosphate (according to UniProt)
[wiki|Domains]
[wiki|ATP-grasp domain] (aa 120-318) (according to UniProt)
Biotin carboxylation domain (aa 1-447) (according to UniProt)
Structure
[PDB|1BNC] (the protein from ''E. coli'') [Pubmed|7915138]
Paralogous protein(s)
[protein|5C8815965146BCD8ED238258B3F820AA17241165|yotE]
Expression and Regulation
Operons
genes
[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
description
[Pubmed|7592499]
Open in new tab

[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]→[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]

2021-09-18 05:52:38

ghost

13

86e257ca959326e8b837637c9b51bc6f18c4c8c4

C4E0BC3C47DE59CA027C5A57E3491771B42AECD9

Biological materials
Expression vectors
GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP1025, N-terminal Strep-tag, based on [wiki|pGP172], purification from ''E. coli'', available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1483 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1491 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
15952903,12121720,15968460
Original Publications
7592499,7915138,28579978,28516784,33481774

1DFFEB155028A5C6B4B344769EF7DAAC14983322

Page visits: 2272

Time of last update: 2021-09-19 02:23:31

Author of last update: Jstuelk