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accA
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accA
168

acetyl-CoA carboxylase (alpha subunit)

locus
BSU_29200
Molecular weight
36.18 kDa
pI
6.09
Protein length
Gene length
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (alpha subunit))
essential
yes
ec
6.4.1.2
synonyms
accA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0825

This gene is a member of the following regulons

Gene
Coordinates
2,987,731  2,988,708
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
accA family (single member, according to UniProt)
[wiki|Domains]
CoA carboxyltransferase C-terminal domain (aa 38-292) (according to UniProt)
Structure
[PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], 64% identity) [Pubmed|16460018]
[wiki|Localization]
cytoplasm (according to Swiss-Prot),  Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
description
[Pubmed|22383849]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
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[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]→[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]

2021-09-23 05:51:17

ghost

10

b652df2692748c745aab0f8ceb0e50c1ffcb2a82

36A9759B4AA4F3ACBABBC425410BB00129DBEA9D

Biological materials
Expression vectors
pGP1021: purification from ''E. coli'' with an N-terminal His-tag, with TEV cleavage site, (in [wiki|pETM-11]), available in [wiki|Jörg Stülke]'s lab
pGP1024: purification from ''E. coli'' with an N-terminal Strep-tag, with TEV cleavage site, (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1723: purification from ''B. subtilis'' with an C-terminal Strep-tag, for [wiki|SPINE], (in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1487 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1490 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
15952903,17919287,12121720,15968460
Original Publications
15066985,16460018,14651647,16479537,12663926,22383849,28579978,16460018,27471863,33481774

22B3039DF4D106F121DBC765F49878C9B324FDB2

Page visits: 2957

Time of last update: 2021-09-23 18:51:50

Author of last update: Jstuelk