gcaD

gcaD
168

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

locus
BSU_00500
Molecular weight
49.29 kDa
pI
5.65
Protein length
Gene length
function
cell wall metabolism
product
bifunctional N-acetylglucosamine-1-phosphate
essential
yes
ec
2.7.7.23
synonyms
gcaD, tms, tms26, glmU

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1207

This gene is a member of the following regulons

Gene
Coordinates
56,352  57,722
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + α-D-glucosamine 1-phosphate --> CoA + H+ + N-acetyl-α-D-glucosamine 1-phosphate (according to UniProt)
H+ + N-acetyl-α-D-glucosamine 1-phosphate + UTP --> diphosphate + UDP-N-acetyl-α-D-glucosamine (according to UniProt)
Protein family
N-terminal domain: N-acetylglucosamine-1-phosphate uridyltransferase family (single member, according to UniProt)
C-terminal domain: [wiki|Transferase hexapeptide repeat family] (according to UniProt)
Structure
[PDB|1HM9] (from ''Streptococcus pneumoniae'', 49% identity) [Pubmed|11118459]
[AF|P14192]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
description
[Pubmed|22383849]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|8522540], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]→[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]

2022-11-27 08:24:39

Jstuelk

50

8a2ec596272c7cfb42cb975d3b44431fc04457cf

58FF1AAE3D8324581B04A0D2CE6BF74D3CC7B05C

Biological materials
Expression vectors
pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
References
1328164,8522540,11118459,21303765

23540C0D84F55135E39A09F48E48AB6F7C270B2E

Page visits: 3820

Time of last update: 2022-11-28 23:14:11

Author of last update: Melvin.boenninger