mraY
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mraY
168

phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate), catalyzes the first commited membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I

locus
BSU_15190
Molecular weight
35.44 kDa
pI
8.97
Protein length
Gene length
function
peptidoglycan precursor biosynthesis
product
phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate)
essential
yes
ec
2.7.8.13
synonyms
mraY

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0472

This gene is a member of the following regulons

Gene
Coordinates
1,587,926  1,588,900
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine --> Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol + UMP (according to UniProt)
Protein family
glycosyltransferase 4 family (with [protein|D9257531C2E24426781342314953B89A8272C5E5|tagO], according to UniProt)
Structure
[PDB|5JNQ] [Pubmed|28068312]
[AF|Q03521]
[wiki|Localization]
cell membrane (according to UniProt)
forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [Pubmed|20233306]
Expression and Regulation
Operons
genes
[gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
description
[Pubmed|1391053]
regulation
expressed during vegatative growth [Pubmed|1391053]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8320223], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
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[gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]→[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

2022-08-12 09:07:34

Jstuelk

24

68c6899b3f1263bd636c35a4ea9512c00078a714

2F040145AC628A38E3D11955965F4DA256DE6790

Biological materials
FLAG-tag construct
GP2001 ''mraY-3xFLAG ermC'' (based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
References
15131133,8436954,18791230,18081839,7734839,21937437,18672909,20537545,10790316,1391053,24895118,25127465,26620564,20233306,27226570,27312048,27531195,28068312,30395652

4D3D4429E1F387BACB0D5A1084AA8DF179A16685

Page visits: 4726

Time of last update: 2022-08-16 20:16:36

Author of last update: Melvin.boenninger