ahrC
168
transcriptional regulator of the ArgR family, AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism
locus
BSU_24250
Molecular weight
16.69 kDa
pI
5.52
function
transcriptional regulator of arginine metabolic genes
product
transcriptional regulator (ArgR family)
essential
no
synonyms
ahrC, argR
Genomic Context
Categories containing this gene/protein
This gene is a member of the following regulons
Gene
Coordinates
2,522,324 2,522,773
Phenotypes of a mutant
increased intracellular concentration of ornithine, citrulline, and arginine [pubmed|28679749]
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] inactivation allows growth of a [gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA] [gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB] [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA] mutant at low potassium concentration [pubmed|28679749]
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] inactivation allows growth of a [gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA] mutant under conditions of osmotic stress (0.8 M NaCl) [pubmed|35783388]
The protein
Catalyzed reaction/ biological activity
transcriptional activator/ repressor of genes involved in arginine metabolism
Protein family
ArgR family (single member, according to UniProt)
[wiki|Cofactors]
L-arginine is the co-factor required for transcription repression/ activation [pubmed|18007040]
Structure
[PDB|2P5L] (complex with an 18bp DNA operator) [pubmed|18455186]
[PDB|2P5K] (N-terminus) [pubmed|18007039]
[PDB|2P5M] (C-Terminus) [pubmed|18007040]
[PDB|1F9N] [pubmed|11856827]
[AF|P17893]
Effectors of protein activity
L-arginine is the co-factor required for transcription repression/ activation [pubmed|18007040]
[wiki|Localization]
Cytoplasm
Expression and Regulation
Operons
genes
[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[Pubmed|2106508]
regulation
by [protein|search|sRNA] [protein|search|sr1]
additional information
expression is fourfold increased upon depletion of ''[wiki|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]
genes
[gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
Other regulations
[protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]: translation inhibition
additional information
the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA controls [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression via binding to the [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA, binding is enhanced by [gene|93F328524989597C7D2329B25E665496C9631E87|csrA] [pubmed|31043113]
Biological materials
Mutant
GP729 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
GP3122 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''phleo''), available in [wiki|Jörg Stülke]'s lab
GP2185 (''Δ''''[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]''::''ermC''), available in [wiki|Jörg Stülke]'s lab [pubmed|28679749]
BKE24250 ([gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAGCACCTCTATTTC, downstream forward: _UP4_TAACAGAAAATCTAACAAAG
BKK24250 ([gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAGCACCTCTATTTC, downstream forward: _UP4_TAACAGAAAATCTAACAAAG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Simon Phillips], Leeds University, UK [http://www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=SEVP Homepage]
[wiki|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
References
Reviews
Original Publications
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Time of last update: 2023-04-01 08:16:41
Author of last update: Jstuelk