At Gram+ 2022 in Prague, there will be a workshop on how to get the most out of SubtiWiki. We will present newly added features. Moreover, we hope to learn from you what you would like to have added to SubtiWiki to improve it!
proG
168
1-pyrroline-5-carboxylate dehydrogenase
locus
BSU_12910
Molecular weight
30.05 kDa
pI
4.91
function
biosynthesis of proline
product
1-pyrroline-5-carboxylate dehydrogenase
essential
no
ec
1.5.1.2
synonyms
proG, ykeA, yzcA
outlinks
Genomic Context
Categories containing this gene/protein
This gene is a member of the following regulons
Gene
Coordinates
1,359,454 1,360,272
The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+ [pubmed|28824574]
L-proline + NADP+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADPH (according to UniProt)
L-proline + NAD+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADH (according to UniProt)
Protein family
[wiki|pyrroline-5-carboxylate reductase family] [pubmed|28824574]
[wiki|Cofactors]
NADPH [pubmed|28824574]
Structure
[AF|Q00777]
Effectors of protein activity
feedback-inhibited by proline [pubmed|28824574]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]
description
[Pubmed|1766370,21784929]
Biological materials
Mutant
MGNA-B308 (ykeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1307 NBRP B. subtilis, Japan]
BKE12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
BKK12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
References
Reviews
Original Publications
Page visits: 886
Time of last update: 2022-05-17 15:30:31
Author of last update: Melvin.boenninger