proG
168
1-pyrroline-5-carboxylate dehydrogenase
locus
BSU_12910
Molecular weight
30.05 kDa
pI
4.91
function
biosynthesis of proline
product
1-pyrroline-5-carboxylate dehydrogenase
essential
no
ec
1.5.1.2
synonyms
proG, ykeA, yzcA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,359,454 1,360,272
The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+ [pubmed|28824574]
L-proline + NADP+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADPH (according to UniProt)
L-proline + NAD+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADH (according to UniProt)
Protein family
[wiki|pyrroline-5-carboxylate reductase family] [pubmed|28824574]
[wiki|Cofactors]
NADPH [pubmed|28824574]
Structure
[AF|Q00777]
Effectors of protein activity
feedback-inhibited by proline [pubmed|28824574]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]
description
[Pubmed|1766370,21784929]
Biological materials
Mutant
MGNA-B308 (ykeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1307 NBRP B. subtilis, Japan]
BKE12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
BKK12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
GP3728 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::tet trpC2) available in the [wiki|Stülke] lab
GP3741 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::aphA3 trpC2) available in the [wiki|Stülke] lab
Expression vectors
pGP3791: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP3863: expression of Strep-''proG'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Page visits: 2320
Time of last update: 2025-07-10 02:18:28
Author of last update: Robert.warneke