iolA
168
methylmalonate-semialdehyde dehydrogenase (acylating)
locus
BSU_39760
Molecular weight
53.29 kDa
pI
5.14
function
myo-inositol catabolism
product
methylmalonate-semialdehyde dehydrogenase (acylating)
essential
no
ec
1.2.1.27
synonyms
iolA, mmsA, yxdA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,082,920 4,084,383
The protein
Catalyzed reaction/ biological activity
NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
3-oxopropanoate + CoA + NAD+ --> acetyl-CoA + CO2 + NADH (according to UniProt)
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ --> H+ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)
Protein family
[wiki|aldehyde dehydrogenase family] (according to UniProt)
[wiki|Cofactors]
Coenzyme A, NAD [Pubmed|22782904]
Structure
[PDB|1T90]\t [Pubmed|22782904]
[AF|P42412]
Paralogous protein(s)
[protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]
Expression and Regulation
Operons
genes
[gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
description
[Pubmed|9226270]
regulation
induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]
regulatory mechanism
[protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR]: repression, [Pubmed|9887260,9226270], in [regulon|protein:5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9226270], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B770 (iolA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1769 NBRP B. subtilis, Japan]
BKE39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
BKK39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
labs
[wiki|Yasutaro Fujita], University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
Page visits: 5724
Time of last update: 2025-07-15 23:35:20
Author of last update: Melvin.boenninger