At Gram+ 2022 in Prague, there will be a workshop on how to get the most out of SubtiWiki. We will present newly added features. Moreover, we hope to learn from you what you would like to have added to SubtiWiki to improve it!
iolA
168
methylmalonate-semialdehyde dehydrogenase (acylating)
locus
BSU_39760
Molecular weight
53.29 kDa
pI
5.14
function
myo-inositol catabolism
product
methylmalonate-semialdehyde dehydrogenase (acylating)
essential
no
ec
1.2.1.27
synonyms
iolA, mmsA, yxdA
outlinks
Genomic Context
Categories containing this gene/protein
This gene is a member of the following regulons
Gene
Coordinates
4,082,920 4,084,383
The protein
Catalyzed reaction/ biological activity
NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
3-oxopropanoate + CoA + NAD+ --> acetyl-CoA + CO2 + NADH (according to UniProt)
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ --> H+ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)
Protein family
[wiki|aldehyde dehydrogenase family] (according to UniProt)
[wiki|Cofactors]
Coenzyme A, NAD [Pubmed|22782904]
Structure
[PDB|1T90]\t [Pubmed|22782904]
[AF|P42412]
Paralogous protein(s)
[protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]
Expression and Regulation
Operons
genes
[gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
description
[Pubmed|9226270]
regulation
induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]
regulatory mechanism
[protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR]: repression, [Pubmed|9887260,9226270], in [regulon|protein:5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9226270], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B770 (iolA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1769 NBRP B. subtilis, Japan]
BKE39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
BKK39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA, downstream forward: _UP4_TAAACGACGAACAGCCGAAC
labs
[wiki|Yasutaro Fujita], University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
Page visits: 1882
Time of last update: 2022-05-24 20:30:34
Author of last update: Melvin.boenninger