sasB

sasB
168

(p)ppGpp synthetase, contributes to cellular heterogeneity in protein synthesis during nutrient limitation

locus
BSU_11600
Molecular weight
24.56 kDa
pI
6.71
Protein length
Gene length
function
ppGpp synthesis independent from stringent response
product
(p)ppGpp synthetase
essential
no
synonyms
sasB, yjbM, relQ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2357

This gene is a member of the following regulons

Gene
Coordinates
1,237,006  1,237,641
Phenotypes of a mutant
a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of [gene|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB] or inactivation of [gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|24163341]
a [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] [gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] triple mutant acquires suppressor mutations in [gene|AC67305E4C165CC1D785E49B7CD8335346134376|guaA], [gene|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB], [gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk] or inactivation of [gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] [Pubmed|24682323,24163341]
The protein
Catalyzed reaction/ biological activity
ATP + GTP --> AMP + guanosine 3'-diphosphate 5'-triphosphate (according to UniProt)
Protein family
RelA/SpoT family (with [protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] and [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel], according to UniProt)
[wiki|Domains]
single ppGpp synthetase domain [Pubmed|24163341]
Structure
[PDB|5DEC] [Pubmed|26460002]
[AF|O31611]
Effectors of protein activity
allosterically activated by pppGpp [Pubmed|26460002], this is inhibited by the [protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] product pGpp [pubmed|35594298]
Paralogous protein(s)
[protein|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA]
Expression and Regulation
Operons
genes
[gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
description
[Pubmed|18067544]
regulation
expressed during exponential growth
Open in new tab

[gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]→[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]

2022-12-08 06:52:45

Jstuelk

33

c5edd61e714281007d4d9ec99a1efa0cfef63f09

83E46551CBC32AB07130281546FE3D422612C2C3

Biological materials
Mutant
MGNA-B159 (yjbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1158 NBRP B. subtilis, Japan]
GP2066 ([gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA] [gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB] [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel]::mls), available in [wiki|Jörg Stülke]'s lab
BKE11600 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC,  downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
BKK11600 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11600 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC,  downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
GP3470 (Δ[gene|1107A963C253FBEE8716507BD0EC0E7CD5642B12|sasA]::spec Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo), available in [wiki|Jörg Stülke]'s lab
GP3472 (Δ[gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel]::cat Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo), available in [wiki|Jörg Stülke]'s lab
GP3425 (Δ[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]::phleo) , available in [wiki|Jörg Stülke]'s lab
GP3467 ([gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-6xHis-cat), available in [wiki|Jörg Stülke]'s lab
Expression vectors
pGP3452 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP3693 (based on [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
27149325,30980074,33690958
Original Publications
22950019,18670626,19447912,18067544,24163341,24489751,24682323,26460002,27875634,35594298

81BCF5C08EC1A580F5E05884BA830130FC7E46E9

Page visits: 3158

Time of last update: 2022-12-07 18:18:55

Author of last update: Jstuelk