ispE

ispE
168

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis

locus
BSU_00460
Molecular weight
31.57 kDa
pI
6.35
Protein length
Gene length
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
ispE, yabH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1947

This gene is a member of the following regulons

Gene
Coordinates
53,516  54,385
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (according to UniProt)
4-CDP-2-C-methyl-D-erythritol + ATP --> 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+ (according to UniProt)
Protein family
GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK] and [protein|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]) (according to UniProt)
Structure
[PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]
[AF|P37550]
Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulation
negative autoregulation [Pubmed|7638212]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
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[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]→[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]

2022-12-01 02:48:42

Jstuelk

35

620be59868c14a2ea15f75e6fdf1022628ca65bb

B42ADE39EB92F53783BD0BDEF129E37859FEBF47

Biological materials
Mutant
MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]
References
17458547,23840410,12771135,29335298

8FA635D1915108BF29A864AF4DA28ECF633F735F

Page visits: 3134

Time of last update: 2022-12-01 09:35:59

Author of last update: Melvin.boenninger