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gabD

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gabD
168

succinate-semialdehyde dehydrogenase (NADP), general stress protein

locus
BSU_03910
Molecular weight
50.10 kDa
pI
4.76
Protein length
Gene length
function
utilization of gamma-amino butyric acid
product
succinate-semialdehyde dehydrogenase (NADP)
essential
no
synonyms
gabD, ycnH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1012

This gene is a member of the following regulons

Gene
Coordinates
442,950  444,338
Phenotypes of a mutant
growth defect in the presence of gamma-aminobutyrate  [Pubmed|24127574]
The protein
Catalyzed reaction/ biological activity
H2O + NADP+ + succinate semialdehyde --> 2 H+ + NADPH + succinate (according to UniProt)
Protein family
[wiki|aldehyde dehydrogenase family] (according to UniProt)
Structure
[PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 36% identity, 68% similarity)
Paralogous protein(s)
[protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH]
Expression and Regulation
Operons
genes
[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]
description
[Pubmed|12123465]
regulation
''[protein|search|gabD]'': induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR]: activation, [Pubmed|12123465], in [regulon|protein:C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12123465], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Open in new tab

[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]

2021-10-04 18:32:11

ghost

4

3a0a53baa6f2174a955534e2b8ea518fd527da6e

1DE87C14F04B6A836A6E076AE45BB40C9B60A561

genes
[gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]-[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]
description
[Pubmed|12123465]
regulation
''[protein|search|gabD]'': induced by stress ([protein|search|SigB]) [Pubmed|15805528]
regulatory mechanism
[protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR]: activation, [Pubmed|32147931,12123465], in [regulon|protein:C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12123465], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]→[gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]

2021-11-21 08:36:11

ghost

9

e3e4e1f1822033b4069ae3a9c7bc374af762e4a0

3807B263B7F7E1F259288EF64815902EECED7726

Biological materials
Mutant
MGNA-C015 (ycnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2013 NBRP B. subtilis, Japan]
BKE03910 ([gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]::erm  trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGAACATTCCTTTCT,  downstream forward: _UP4_TAAAAGAATGCACGCTCCTG
BKK03910 ([gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]::kan  trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGAACATTCCTTTCT,  downstream forward: _UP4_TAAAAGAATGCACGCTCCTG
labs
[wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
References
15223311,12123465,11544224,15805528,28516784

A0BEB92D54799956A4ADE106A1388E5710141069

Page visits: 2744

Time of last update: 2022-01-20 15:02:47

Author of last update: Melvin.boenninger