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accB
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accB
168

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

locus
BSU_24350
Molecular weight
17.09 kDa
pI
4.39
Protein length
Gene length
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
essential
yes
ec
6.4.1.2
synonyms
accB, fabE, yqhW

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0511

This gene is a member of the following regulons

Gene
Coordinates
2,531,718  2,532,197
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
[wiki|Domains]
Biotinyl-binding domain (aa 81-157) (according to UniProt)
[wiki|Cofactors]
biotin [Pubmed|24816803]
Structure
[PDB|1BDO] (the protein from ''E. coli'') [Pubmed|8747466]
Additional information
AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [wiki|SPINE].
Expression and Regulation
Operons
genes
[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
description
[Pubmed|7592499]
Open in new tab

[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]→[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]

2021-09-18 05:52:38

ghost

13

86e257ca959326e8b837637c9b51bc6f18c4c8c4

C4E0BC3C47DE59CA027C5A57E3491771B42AECD9

Biological materials
Expression vectors
GP1477 (chromosomal ''accB''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP1027: IPTG inducible expression, purification in ''E. coli'' without any tag, in [wiki|pGP574], available in [wiki|Jörg Stülke]'s lab
pGP2649: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP2650: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP2652: IPTG inducible expression, purification in ''E. coli'' with C-terminal Strep-tag, in [wiki|pGP574], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1484 (''mls'', based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1493 (mls, based on [wiki|pGP1080]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
15952903,12121720,15968460
Original Publications
7592499,8747466,24816803,28579978,31113899

ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22

Page visits: 2936

Time of last update: 2021-09-23 15:10:20

Author of last update: Melvin.boenninger